Inducer-dependent conditional-lethal mutant animal viruses.

Inducer - dependent conditional - lethal mutant animal viruses ( lac operator / lac repressor protein / vaccinia virus )

Regulatory elements of the Escherichia coli lac operon were used to construct an inducer-dependent conditional-lethal mutant animal virus. The gene encoding the repressor protein of the lac operon was integrated into the vaccinia virus genome so that it was expressed constitutively, and the lac operator was inserted next to the promoter ofa gene that encodes an 11-kDa virion-associated protein ...

A new Overlay for Plaquing Animal Viruses

A Chinese hamster ovary cell mutant with a heat-sensitive, conditional- lethal defect in vacuolar function

We describe a mutant derived from Chinese hamster ovary cells that is offt-sensitive for viability and for resistance to certain protein toxins. This mutant, termed G.7.1, grows normally at 34 degrees C but does not grow in Dulbecco's modified Eagle's medium at 39.5 degrees C. However, when this medium is supplemented with FeSO4, the mutant cells will grow at the elevated temperature. At 39.5 d...

A Nutritional Conditional Lethal Mutant Due to Pyridoxine 5′-Phosphate Oxidase Deficiency in Drosophila melanogaster

The concept of auxotrophic complementation has been proposed as an approach to identify genes in essential metabolic pathways in Drosophila melanogaster. However, it has achieved limited success to date, possibly due to the low probability of finding mutations fit with the chemically defined profile. Instead of using the chemically defined culture media lacking specific nutrients, we used bare ...

A Nutritional Conditional Lethal Mutant Due to Pyridoxine 59-Phosphate Oxidase Deficiency in Drosophila melanogaster

The concept of auxotrophic complementation has been proposed as an approach to identify genes in essential metabolic pathways in Drosophila melanogaster. However, it has achieved limited success to date, possibly due to the low probability of finding mutations fit with the chemically defined profile. Instead of using the chemically defined culture media lacking specific nutrients, we used bare ...